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钙离子荧光探针Fluo-5F,AM

产品基本信息

货号:20560

产品名称:钙离子荧光探针Fluo-5F, AM

规格:10x50 ug

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1100.91

溶剂:DMSO

激发波长(nm):494

发射波长(nm):516

 

适用仪器


流式细胞仪 
激发:488nm激发
发射:530/30nm滤波片
通道:FITC通道
荧光显微镜 
激发:FITC
发射:FITC
推荐孔板:黑色透明
荧光酶标仪 
激发:490nm
发射:525nm
cutoff:515nm
推荐孔板:黑色透明
读取模式:底读模式

 

产品介绍

钙离子荧光探针Fluo-5F, AM是美国AAT Bioquest生产的钙离子荧光探针,Fluo-5F是Fluo-4的类似物,具有较低的钙结合亲和力(Kd =〜2.3 uM),使其适合检测1 µM至1 mM的细胞内钙水平,该水平会饱和Fluo-4的响应。通过将溶解的指示剂直接添加到含有培养细胞的培养皿中,可以在细胞中加载Fluo-5F AM酯。它与氩离子激光源在488 nm处激发兼容,使Fluo-5F可用于共聚焦显微镜,流式细胞仪和微孔板筛选应用。它的激发和发射波长分别为494和516 nm。钙结合后,其荧光强度增加> 100倍。齐岳生物是AAT Bioquest的中国代理商,为您提供*优质的钙离子荧光探针Fluo-5F, AM。 

点击查看光谱

点击查看实验方案

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signalingAuthors: Yanjiao Wu, Xiaoli Xu, Lunkun Ma, Qian Yi, Weichao Sun, Liling TangJournal: The International Journal of Biochemistry & Cell Biology (2017)

Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neuronsAuthors: Jia Liu, Qing Du, He Zhu, Yu Li, Maodong Liu, Shoushui Yu, Shilei WangJournal: Int J Clin Exp Med (2017): 6861--6868

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cellsAuthors: Jiang Lu, Xue-qin Yao, Xin Luo, Yu Wang, Sookja Kim Chung, He-xin Tang, Chi Wai Cheung, Xian-yu Wang, Chen Meng, Qing LiJournal: Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogelsAuthors: Gang Yang, Zhenghua Xiao, Xiaomei Ren, Haiyan Long, Kunlong Ma, Hong Qian, Yingqiang GuoJournal: Scientific Reports (2017): 41781

Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protectionAuthors: Xia Sun, Yi Lin, Qiansheng Huang, Junpeng Shi, Ling Qiu, Mei Kang, Yajie Chen, Chao Fang, Ting Ye, Sijun DongJournal: Journal of cellular and molecular medicine (2015): 581--594

The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia/reperfusion injuryAuthors: Lantao Zhao, Shuhong Li, Shilei Wang, Ning Yu, Jia LiuJournal: Biochemical and biophysical research communications (2015): 537--542

Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.Authors: Xu-Dong Peng, Gui-Qiu Zhao, Jing Lin, Nan Jiang, Qiang Xu, Cheng-Cheng Zhu, Jain-Qiu Qu, Lin Cong, Hui LiJournal: International journal of ophthalmology (2014): 441--447

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cellsAuthors: Qing Li, Jiang Lu, Xianyu WangJournal: Neural regeneration research (2014): 2002

Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living ratsAuthors: Nan Liang, Peng Wang, Shilei Wang, Shuhong Li, Yu Li, Jinying Wang, Min WangJournal: Journal of Neural Transmission (2014): 593--600

Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve--mast cell interaction in a hapten-induced mouse model of atopic dermatitisAuthors: M Hagiyama, T Inoue, T Furuno, T Iino, S Itami, M Nakanishi, H Asada, Y Hosokawa, A ItoJournal: British Journal of Dermatology (2013): 771--778

 

相关产品

产品名称货号
钙离子荧光探针Cal-520FF, AMQ-21142
钙离子荧光探针Fluo-8FF, AMQ-21104

西安齐岳生物科技有公司自己的实验室合成荧光标记的蛋白,糖,氨基酸,高分子等产品,我们可以提供的荧光染料包括有以下不同波段的荧光染料产品,荧光染料包括以下荧光染料FITC/Rhodamine/TRITC/Comarin/Bodipy/ICG/CY3/CY3.5/CY5/CY5.5/CY7/CY7.5等等。

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本文由齐岳Lh整理,2022-07-25 09:55:14

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