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trFluorTb羊抗兔免疫球蛋白(H+L)*交叉亲和降低干扰*

产品基本信息

货号:16848

产品名称:trFluor Tb羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰*

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):330

发射波长(nm):544

 

产品介绍

trFluor Tb羊抗兔免疫球蛋白(H+L)是美国AAT Bioquest生产的荧光标记二抗,存在于细胞,血清或其他生物流体中的许多生物化合物都是天然荧光的,因此,由于要测定的生物分子的自发荧光引起的高背景,使用常规的快速荧光团会严重限制测定灵敏度。长寿命荧光团与时间分辨检测(激发和发射检测之间的延迟)结合使用,可*大程度地减少即时荧光干扰。我们的trFluor Tb探针可用于需要高灵敏度的分析的时间分辨荧光(TRF)。与更传统的荧光团(例如Alexa Fluor或花青染料)相比,trFluor Tb探针具有较大的斯托克斯位移和极长的发射半衰期。与其他TRF化合物相比,我们的trFluor Tb探针具有相对较高的稳定性,高发射率和与生物分子连接的能力。当与一抗结合使用时,这种trFluor Tb山羊抗兔IgG(H + L)缀合物通常用作间接免疫荧光染色的第二步试剂。齐岳生物是AAT Bioquest的中国代理商,为您提供*优质的trFluor Tb羊抗兔免疫球蛋白(H+L)。 

点击查看光谱

 

参考文献

Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitorsAuthors: Lo MC, Ngo R, Dai K, Li C, Liang L, Lee J, Emkey R, Eksterowicz J, Ventura M, Young SW, Xiao SH.Journal: Anal Biochem (2012): 368

Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase AssaysAuthors: Saville L, Spais C, Mason JL, Albom MS, Murthy S, Meyer SL, Ator MA, Angeles TS, Husten J.Journal: Assay Drug Dev Technol. (2012)

A homogeneous single-label time-resolved fluorescence cAMP assayAuthors: Martikkala E, Rozwandowicz-Jansen A, Hanninen P, Petaja-Repo U, Harma H.Journal: J Biomol Screen (2011): 356

Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1aAuthors: Leyris JP, Roux T, Trinquet E, Verdie P, Fehrentz JA, Oueslati N, Douzon S, Bourrier E, Lamarque L, Gagne D, Galleyrand JC, M'Kadmi C, Martinez J, Mary S, Baneres JL, Marie J.Journal: Anal Biochem (2011): 253

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approachAuthors: Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A.Journal: J Phys Chem B (2011): 11439

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodiesAuthors: Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.Journal: J Biol Chem (2011): 11337

A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerizationAuthors: Kota S, Scampavia L, Spicer T, Beeler AB, Takahashi V, Snyder JK, Porco JA, Hodder P, Strosberg AD.Journal: Assay Drug Dev Technol (2010): 96

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRETAuthors: Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G.Journal: J Biol Chem (2010): 23318

Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformationAuthors: Posokhov YO, Kyrychenko A, Ladokhin AS.Journal: Anal Biochem (2010): 284

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairsAuthors: Visser AJ, Laptenok SP, Visser NV, van Hoek A, Birch DJ, Brochon JC, Borst JW.Journal: Eur Biophys J (2010): 241

 

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ICG是一种特殊的荧光染料,可被波长范围在750~810 nm的外来光所激发,发射波长约840 nm的近红外光。局部注射的ICG可被淋巴系统吸收并与淋巴系统中的白蛋白结合,随淋巴系统引流至淋巴结*终回流至血液系统。由于淋巴系统转运缓慢,ICG可在淋巴系统内存在较长时间,ICG荧光成像技术正是基于以上原理,通过特殊的显像设备实现引流淋巴管和淋巴结的示踪。并且由于不同组织摄取ICG率不同,其可在术中分辨淋巴组织与胃周血管、脂肪、胰腺等其他组织。

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本文由齐岳Lh整理,2022-07-26 09:48:00

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